Choose to “Remove new trimmed regions from sequences” and set the Error probability limit to 0.01, as shown in the screenshot below. Click OK and then Save once the trimming is finished.įrom here it is more efficient to finish cleaning up and editing the sequences once they are aligned. Select the sequence list (Cyanistes CR sequences) again and click Align/Assemble→Multiple Align. You can view the chromatograms while browsing through folders Trim low quality ends automatically.ĭNA Chromatogram Explorer Lite is a Windows Explorer clone dedicated to DNA sequence analysis and manipulation. With a single click you can trim the low quality bases at the end of your samples. The Lite version of Chromatogram Explorer is freeware. Start DNA Chromatogram Explorer and navigate to your DNA sample files (chromatograms).Īll chromatograms in that folder will be displayed in the right panel (see picture below). SCF and ABI (ABI, AB, AB1, AB!) chromatogram files are supported. Low quality ends are shown in dark gray color. To view non-chromatogram files (FASTA, SEQ, TXT) just double click them. Press 'Convert' or 'Convert all' and your file will be saved as SCF or FASTA (as you choose). You can automatically trim low quality ends of all chromatograms in a folder. Please see this short tutorial.ĭNA Chromatogram Explorer is delivered in a small package together with other free molecular biology tools. You don't need administrator rights in order to 'install' this package.
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